TG004238

Peptide	R.EGVFVSN[H5N5F]GTHWFVTQR.N (PE001614)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H5N5F (N7)
Mod(s)
Molecular Weight	3,852.636
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
31.894	31.878	44.837	1,279.216	3+	1,735.754	2+	Y[pH4N4F]	68.1
31.894	31.878	44.837	1,279.216	3+	1,662.725	2+	Y[pH4N4]	25.5
31.894	31.878	44.837	1,279.216	3+	1,654.728	2+	Y[pH3N4F]	20.1
31.894	31.878	44.837	1,279.216	3+	1,472.162	2+	Y[pH2N3F]	9.3
31.894	31.878	44.837	1,279.216	3+	1,399.133	2+	Y[pH2N3]	8.8
31.894	31.878	44.837	1,279.216	3+	1,480.159	2+	Y[pH3N3]	8.4
31.894	31.878	44.837	1,279.216	3+	1,581.699	2+	Y[pH3N4]	8.1
31.894	31.878	44.837	1,279.216	3+	1,816.781	2+	Y[pH5N4F]	7.1
31.894	31.878	44.837	1,279.216	3+	1,034.001	2+	Y[pN]	6.5
31.894	31.878	44.837	1,279.216	3+	1,135.540	2+	Y[pN2]	6.0

Spectra

6234;EThcD;31.885;6226;1279.225;Byonic:q-value=0.00104/GPS:MinICScore=1.944
6236;HCD;31.894;6226;1279.225;