TG004222

Peptide	R.EGVFVSN[H4N5]GTHWFVTQR.N (PE001531)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H4N5 (N7)
Mod(s)
Molecular Weight	3,544.525
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.348	32.273	45.820	1,176.513	3+	1,581.699	2+	Y[pH3N4]	59.4
32.348	32.273	45.820	1,176.513	3+	1,662.725	2+	Y[pH4N4]	43.1
32.348	32.273	45.820	1,176.513	3+	1,480.159	2+	Y[pH3N3]	28.7
32.348	32.273	45.820	1,176.513	3+	1,399.133	2+	Y[pH2N3]	27.7
32.348	32.273	45.820	1,176.513	3+	1,034.001	2+	Y[pN]	13.7
32.348	32.273	45.820	1,176.513	3+	1,500.673	2+	Y[pH2N4]	11.9
32.348	32.273	45.820	1,176.513	3+	1,297.593	2+	Y[pH2N2]	10.7
32.348	32.273	45.820	1,176.513	3+	1,135.540	2+	Y[pN2]	9.6
32.348	32.273	45.820	1,176.513	3+	1,378.620	2+	Y[pH3N2]	7.2
32.348	32.273	45.820	1,176.513	3+	1,216.567	2+	Y[pHN2]	6.2
32.348	32.273	45.820	1,176.513	3+	1,318.106	2+	Y[pHN3]*	5.1

Spectra

6294;EThcD;32.169;6288;1176.522;Byonic:q-value=0.00104/GPS:MinICScore=14.554
6296;HCD;32.178;6288;1176.522;
6367;EThcD;32.511;6360;1176.854;Byonic:q-value=0.00142/GPS:MinICScore=124.269
6368;HCD;32.517;6360;1176.854;