TG004220

Peptide	R.EGVFVSN[H4N6]GTHWFVTQR.N (PE001511)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H4N6 (N7)
Mod(s)
Molecular Weight	3,747.604
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.261	32.096	45.379	1,244.206	3+	1,764.265	2+	Y[pH4N5]	66.2
32.261	32.096	45.379	1,244.206	3+	1,683.239	2+	Y[pH3N5]	50.3
32.261	32.096	45.379	1,244.206	3+	1,581.699	2+	Y[pH3N4]	30.1
32.261	32.096	45.379	1,244.206	3+	1,500.673	2+	Y[pH2N4]	25.3
32.261	32.096	45.379	1,244.206	3+	1,399.133	2+	Y[pH2N3]	20.4
32.261	32.096	45.379	1,244.206	3+	1,662.725	2+	Y[pH4N4]	15.0
32.261	32.096	45.379	1,244.206	3+	1,034.001	2+	Y[pN]	11.8
32.261	32.096	45.379	1,244.206	3+	1,480.159	2+	Y[pH3N3]	10.1
32.261	32.096	45.379	1,244.206	3+	1,135.540	2+	Y[pN2]	8.3
32.261	32.096	45.379	1,244.206	3+	1,297.593	2+	Y[pH2N2]	7.3
32.261	32.096	45.379	1,244.206	3+	1,216.567	2+	Y[pHN2]	5.2

Spectra

6311;EThcD;32.245;6302;1244.215;Byonic:q-value=0.00104/GPS:MinICScore=17.259
6312;HCD;32.261;6302;1244.215;