TG004214

Peptide	R.EGVFVSN[H5N3]GTHWFVTQR.N (PE001515)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H5N3 (N7)
Mod(s)
Molecular Weight	3,300.419
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.489	32.526	46.449	1,095.144	3+	1,459.646	2+	Y[pH4N2]	83.4
32.489	32.526	46.449	1,095.144	3+	1,378.620	2+	Y[pH3N2]	43.7
32.489	32.526	46.449	1,095.144	3+	1,540.672	2+	Y[pH5N2]*	42.5
32.489	32.526	46.449	1,095.144	3+	1,034.001	2+	Y[pN]	29.6
32.489	32.526	46.449	1,095.144	3+	1,297.593	2+	Y[pH2N2]	21.2
32.489	32.526	46.449	1,095.144	3+	1,216.567	2+	Y[pHN2]	13.9
32.489	32.526	46.449	1,095.144	3+	1,135.540	2+	Y[pN2]	12.1

Spectra

6346;EThcD;32.408;6340;1095.485;Byonic:q-value=0.00399/GPS:MinICScore=71.902
6348;HCD;32.427;6340;1095.485;
6374;EThcD;32.542;6369;1095.151;Byonic:q-value=0.00104/GPS:MinICScore=7.527
6376;HCD;32.551;6369;1095.151;