TG004211

Peptide	R.EGVFVSN[H4N6S]GTHWFVTQR.N (PE001604)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H4N6S (N7)
Mod(s)
Molecular Weight	4,038.7
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
37.670	37.618	59.122	1,341.238	3+	1,683.239	2+	Y[pH3N5]	29.2
37.670	37.618	59.122	1,341.238	3+	1,764.265	2+	Y[pH4N5]	23.2
37.670	37.618	59.122	1,341.238	3+	1,909.813	2+	Y[pH4N5S]	18.5
37.670	37.618	59.122	1,341.238	3+	1,500.673	2+	Y[pH2N4]	16.8
37.670	37.618	59.122	1,341.238	3+	1,581.699	2+	Y[pH3N4]	16.7
37.670	37.618	59.122	1,341.238	3+	1,399.133	2+	Y[pH2N3]	14.3
37.670	37.618	59.122	1,341.238	3+	1,034.001	2+	Y[pN]	9.9
37.670	37.618	59.122	1,341.238	3+	1,662.725	2+	Y[pH4N4]	7.2
37.670	37.618	59.122	1,341.238	3+	1,480.159	2+	Y[pH3N3]	6.3
37.670	37.618	59.122	1,341.238	3+	1,135.540	2+	Y[pN2]	5.9
37.670	37.618	59.122	1,341.238	3+	1,273.544	3+	Y[pH4N5S]	5.0

Spectra

7474;EThcD;37.664;7466;1341.248;Byonic:q-value=0.00104/GPS:MinICScore=10.829
7475;HCD;37.670;7466;1341.248;