TG004199

Peptide	K.VCEFQFCNDPFLGVYYHKNN[H6N6FS2]K.S (PE001642)
Sequence	VCEFQFCNDPFLGVYYHKNNK
Glycans(s)	H6N6FS2 (N20)
Mod(s)	57.021 (C2), 57.021 (C7)
Molecular Weight	5,615.257
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
47.780	47.692	84.192	1,400.326	4+	1,648.023	3+	Y[pH5N5FS]	18.7
47.780	47.692	84.192	1,400.326	4+	1,550.991	3+	Y[pH5N5F]	13.3
47.780	47.692	84.192	1,400.326	4+	1,327.552	4+	Y[pH6N6FS]	7.6
47.780	47.692	84.192	1,400.326	4+	1,375.262	3+	Y[pH3N4F]	6.2
47.780	47.692	84.192	1,400.326	4+	1,745.054	3+	Y[pH5N5FS2]	5.4

Spectra

9564;EThcD;47.388;9553;1400.579;Byonic:q-value=6.63e-05/GPS:MinICScore=129.895
9565;HCD;47.394;9553;1400.579;
9628;EThcD;47.692;9625;1400.831;Byonic:q-value=5.97e-05/GPS:MinICScore=31.525
9630;HCD;47.702;9625;1400.831;
9661;EThcD;47.849;9657;1400.579;Byonic:q-value=8.08e-06/GPS:MinICScore=37.098
9663;HCD;47.859;9657;1400.579;
9685;EThcD;47.959;9681;1400.332;Byonic:q-value=6.66e-05/GPS:MinICScore=20.276
9687;HCD;47.973;9681;1400.332;