TG004151

Peptide	R.EGVFVSN[H5N3]GTHWFVTQR.N (PE001515)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H5N3 (N7)
Mod(s)
Molecular Weight	3,300.419
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.297	32.361	46.227	1,095.144	3+	1,459.646	2+	Y[pH4N2]	75.8
32.297	32.361	46.227	1,095.144	3+	1,540.672	2+	Y[pH5N2]*	39.3
32.297	32.361	46.227	1,095.144	3+	1,378.620	2+	Y[pH3N2]	39.3
32.297	32.361	46.227	1,095.144	3+	1,034.001	2+	Y[pN]	28.5
32.297	32.361	46.227	1,095.144	3+	1,297.593	2+	Y[pH2N2]	22.1
32.297	32.361	46.227	1,095.144	3+	1,216.567	2+	Y[pHN2]	13.2
32.297	32.361	46.227	1,095.144	3+	1,135.540	2+	Y[pN2]	12.3

Spectra

6158;EThcD;32.127;6150;1095.818;Byonic:q-value=0.000751/GPS:MinICScore=288.162
6160;HCD;32.137;6150;1095.818;
6224;EThcD;32.446;6214;1095.150;Byonic:q-value=2.24e-05/GPS:MinICScore=0.447
6226;HCD;32.456;6214;1095.150;