TG004143

Peptide	K.GYHLMSFPQSAPHGVVFLHVTYVPAQEKN[H4N3F]FTTAPAICHDGK.A (PE001542)
Sequence	GYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGK
Glycans(s)	H4N3F (N29)
Mod(s)	57.021 (C37)
Molecular Weight	5,972.744
Charge	7+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
39.439	40.360	66.347	851.686	7+	932.610	6+	Y[pH3N2F]*	41.9
39.439	40.360	66.347	851.686	7+	951.871	5+	Y[pN]	40.9
39.439	40.360	66.347	851.686	7+	827.241	6+	Y[pN2]	19.6
39.439	40.360	66.347	851.686	7+	981.083	5+	Y[pNF]*	17.6
39.439	40.360	66.347	851.686	7+	881.258	6+	Y[pH2N2]	13.3
39.439	40.360	66.347	851.686	7+	908.267	6+	Y[pH3N2]	11.6
39.439	40.360	66.347	851.686	7+	1,189.587	4+	Y[pN]	11.5
39.439	40.360	66.347	851.686	7+	992.487	5+	Y[pN2]	9.7
39.439	40.360	66.347	851.686	7+	793.394	6+	Y[pN]	9.0
39.439	40.360	66.347	851.686	7+	817.737	6+	Y[pNF]*	8.2
39.439	40.360	66.347	851.686	7+	1,057.308	5+	Y[pH2N2]	7.7
39.439	40.360	66.347	851.686	7+	878.592	6+	Y[pHN2F]*	7.1
39.439	40.360	66.347	851.686	7+	935.276	6+	Y[pH4N2]*	5.7

Spectra

7711;EThcD;39.368;7710;851.976;Byonic:q-value=0.00802/GPS:MinICScore=145.999
7713;HCD;39.377;7710;851.976;