TG004139

Peptide	R.EGVFVSN[H5N4FS]GTHWFVTQR.N (PE001506)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H5N4FS (N7)
Mod(s)
Molecular Weight	3,940.652
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
36.936	37.040	57.996	981.668	4+	1,451.649	2+	Y[pH3N2F]*	22.7
36.936	37.040	57.996	981.668	4+	1,634.215	2+	Y[pH4N3F]	20.7
36.936	37.040	57.996	981.668	4+	1,370.622	2+	Y[pH2N2F]*	18.9
36.936	37.040	57.996	981.668	4+	1,553.188	2+	Y[pH3N3F]	17.1
36.936	37.040	57.996	981.668	4+	1,034.001	2+	Y[pN]	14.2
36.936	37.040	57.996	981.668	4+	1,297.593	2+	Y[pH2N2]	12.3
36.936	37.040	57.996	981.668	4+	981.777	3+	Y[pH2N3F]	9.9
36.936	37.040	57.996	981.668	4+	1,107.030	2+	Y[pNF]*	9.7
36.936	37.040	57.996	981.668	4+	1,135.540	2+	Y[pN2]	9.1
36.936	37.040	57.996	981.668	4+	1,378.620	2+	Y[pH3N2]	8.9
36.936	37.040	57.996	981.668	4+	1,089.812	3+	Y[pH4N3F]	6.2
36.936	37.040	57.996	981.668	4+	1,289.596	2+	Y[pHN2F]*	6.1
36.936	37.040	57.996	981.668	4+	1,216.567	2+	Y[pHN2]	5.6

Spectra

7132;EThcD;36.769;7130;981.675;Byonic:q-value=0.000751/GPS:MinICScore=20.086
7134;HCD;36.779;7130;981.675;
7236;EThcD;37.256;7230;981.676;Byonic:q-value=0.000751/GPS:MinICScore=24.655
7238;HCD;37.265;7230;981.676;