TG004131

Peptide	R.EGVFVSN[H4N3F]GTHWFVTQR.N (PE001507)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H4N3F (N7)
Mod(s)
Molecular Weight	3,284.424
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.373	32.383	46.282	1,089.812	3+	1,451.649	2+	Y[pH3N2F]*	43.7
32.373	32.383	46.282	1,089.812	3+	1,532.675	2+	Y[pH4N2F]*	30.3
32.373	32.383	46.282	1,089.812	3+	1,034.001	2+	Y[pN]	18.5
32.373	32.383	46.282	1,089.812	3+	1,370.622	2+	Y[pH2N2F]*	18.5
32.373	32.383	46.282	1,089.812	3+	1,378.620	2+	Y[pH3N2]	17.1
32.373	32.383	46.282	1,089.812	3+	1,297.593	2+	Y[pH2N2]	15.0
32.373	32.383	46.282	1,089.812	3+	1,107.030	2+	Y[pNF]*	10.7
32.373	32.383	46.282	1,089.812	3+	1,216.567	2+	Y[pHN2]	8.5
32.373	32.383	46.282	1,089.812	3+	1,289.596	2+	Y[pHN2F]*	8.5
32.373	32.383	46.282	1,089.812	3+	1,459.646	2+	Y[pH4N2]*	8.4
32.373	32.383	46.282	1,089.812	3+	1,135.540	2+	Y[pN2]	7.0
32.373	32.383	46.282	1,089.812	3+	1,208.569	2+	Y[pN2F]*	6.0

Spectra

6206;EThcD;32.362;6202;1089.818;Byonic:q-value=0.00475/GPS:MinICScore=14.255
6208;HCD;32.373;6202;1089.818;