TG004122

Peptide	R.EGVFVSN[H4N5F]GTHWFVTQR.N (PE001528)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H4N5F (N7)
Mod(s)
Molecular Weight	3,690.583
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
31.706	31.762	44.720	1,225.199	3+	1,654.728	2+	Y[pH3N4F]	19.4
31.706	31.762	44.720	1,225.199	3+	1,634.215	2+	Y[pH4N3F]*	18.1
31.706	31.762	44.720	1,225.199	3+	1,735.754	2+	Y[pH4N4F]	16.9
31.706	31.762	44.720	1,225.199	3+	1,480.159	2+	Y[pH3N3]	16.1
31.706	31.762	44.720	1,225.199	3+	1,662.725	2+	Y[pH4N4]	11.4
31.706	31.762	44.720	1,225.199	3+	1,553.188	2+	Y[pH3N3F]	10.7
31.706	31.762	44.720	1,225.199	3+	1,034.001	2+	Y[pN]	9.8
31.706	31.762	44.720	1,225.199	3+	1,581.699	2+	Y[pH3N4]	9.7
31.706	31.762	44.720	1,225.199	3+	1,297.593	2+	Y[pH2N2]	8.3
31.706	31.762	44.720	1,225.199	3+	1,378.620	2+	Y[pH3N2]	7.7
31.706	31.762	44.720	1,225.199	3+	1,472.162	2+	Y[pH2N3F]	7.1

Spectra

6058;EThcD;31.648;6051;1225.541;Byonic:q-value=0.00475/GPS:MinICScore=5.056
6060;HCD;31.658;6051;1225.541;
6079;EThcD;31.745;6072;1225.205;Byonic:q-value=0.000751/GPS:MinICScore=5.457
6081;HCD;31.755;6072;1225.205;