TG004118

Peptide	K.GYHLMSFPQSAPHGVVFLHVTYVPAQEKN[H5N5F]FTTAPAICHDGK.A (PE001555)
Sequence	GYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGK
Glycans(s)	H5N5F (N29)
Mod(s)	57.021 (C37)
Molecular Weight	6,540.955
Charge	7+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
39.301	37.885	60.121	932.859	7+	1,027.312	6+	Y[pH4N4F]	41.2
39.301	37.885	60.121	932.859	7+	1,159.546	5+	Y[pH3N3F]	13.7
39.301	37.885	60.121	932.859	7+	1,000.303	6+	Y[pH3N4F]	12.4
39.301	37.885	60.121	932.859	7+	1,189.587	4+	Y[pN]	11.8
39.301	37.885	60.121	932.859	7+	1,036.816	6+	Y[pH4N5]	10.2
39.301	37.885	60.121	932.859	7+	1,127.136	5+	Y[pH2N3F]	9.7
39.301	37.885	60.121	932.859	7+	1,138.540	5+	Y[pH2N4]	9.1
39.301	37.885	60.121	932.859	7+	951.871	5+	Y[pN]	7.8
39.301	37.885	60.121	932.859	7+	1,138.817	4+	Y[p]	6.7
39.301	37.885	60.121	932.859	7+	1,097.924	5+	Y[pH2N3]	6.7
39.301	37.885	60.121	932.859	7+	1,240.357	4+	Y[pN2]	6.3
39.301	37.885	60.121	932.859	7+	1,065.514	5+	Y[pHN3]*	6.0
39.301	37.885	60.121	932.859	7+	1,191.957	5+	Y[pH4N3F]	5.7
39.301	37.885	60.121	932.859	7+	992.487	5+	Y[pN2]	5.5
39.301	37.885	60.121	932.859	7+	1,226.102	4+	Y[pNF]*	5.3
39.301	37.885	60.121	932.859	7+	881.258	6+	Y[pH2N2]	5.1
39.301	37.885	60.121	932.859	7+	1,094.725	5+	Y[pHN3F]*	5.1

Spectra

7623;EThcD;38.992;7622;933.005;Byonic:q-value=6.19e-05/GPS:MinICScore=197.632
7625;HCD;39.001;7622;933.005;
7762;EThcD;39.591;7761;933.149;Byonic:q-value=0.000121/GPS:MinICScore=147.593
7764;HCD;39.600;7761;933.149;