TG004104

Peptide	R.EGVFVSN[H7N6S4]GTHWFVTQR.N (PE001509)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H7N6S4 (N7)
Mod(s)
Molecular Weight	5,398.145
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
60.006	60.231	116.328	1,346.041	4+	1,480.159	2+	Y[pH3N3]	12.4
60.006	60.231	116.328	1,346.041	4+	1,034.001	2+	Y[pN]	10.4
60.006	60.231	116.328	1,346.041	4+	1,743.752	2+	Y[pH5N4]	10.0
60.006	60.231	116.328	1,346.041	4+	1,889.300	2+	Y[pH5N4S]	8.5
60.006	60.231	116.328	1,346.041	4+	1,478.611	3+	Y[pH6N5S2]	7.1
60.006	60.231	116.328	1,346.041	4+	1,625.707	2+	Y[pH3N3S]	6.8

Spectra

12110;EThcD;59.224;12101;1346.300;Byonic:q-value=0.000176/GPS:MinICScore=112.630
12111;HCD;59.230;12101;1346.300;
12132;EThcD;59.323;12124;1346.550;Byonic:q-value=0.0001/GPS:MinICScore=90.423
12134;HCD;59.332;12124;1346.550;
12196;EThcD;59.677;12188;1346.300;Byonic:q-value=6.68e-05/GPS:MinICScore=100.811
12197;HCD;59.683;12188;1346.300;
12330;EThcD;60.318;12320;1346.299;Byonic:q-value=1.24e-05/GPS:MinICScore=74.980
12332;HCD;60.328;12320;1346.299;
12377;EThcD;60.542;12366;1346.050;Byonic:q-value=1e-05/GPS:MinICScore=9.147
12379;HCD;60.551;12366;1346.050;
12424;EThcD;60.749;12419;1346.302;Byonic:q-value=9.95e-06/GPS:MinICScore=8.078
12426;HCD;60.759;12419;1346.302;