TG004072

Peptide	R.EGVFVSN[H3N5F]GTHWFVTQR.N (PE001537)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H3N5F (N7)
Mod(s)
Molecular Weight	3,528.53
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.033	32.183	45.779	1,171.181	3+	1,654.728	2+	Y[pH3N4F]	53.1
32.033	32.183	45.779	1,171.181	3+	1,472.162	2+	Y[pH2N3F]	25.5
32.033	32.183	45.779	1,171.181	3+	1,553.188	2+	Y[pH3N3F]	14.2
32.033	32.183	45.779	1,171.181	3+	1,581.699	2+	Y[pH3N4]	10.3
32.033	32.183	45.779	1,171.181	3+	1,399.133	2+	Y[pH2N3]	9.0
32.033	32.183	45.779	1,171.181	3+	1,480.159	2+	Y[pH3N3]	9.0
32.033	32.183	45.779	1,171.181	3+	1,034.001	2+	Y[pN]	6.7
32.033	32.183	45.779	1,171.181	3+	1,573.702	2+	Y[pH2N4F]	5.2
32.033	32.183	45.779	1,171.181	3+	1,297.593	2+	Y[pH2N2]	5.1
32.033	32.183	45.779	1,171.181	3+	1,370.622	2+	Y[pH2N2F]	5.0

Spectra

6135;EThcD;32.023;6127;1171.191;Byonic:q-value=0.000751/GPS:MinICScore=2.713
6137;HCD;32.033;6127;1171.191;