TG003513

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Peptide R.EEQFN[H5N4FS]STYRVVSVLTVLHQDWLNGK.E (PE001406)
Sequence EEQFNSTYRVVSVLTVLHQDWLNGK
Glycans(s) H5N4FS (N5)
Mod(s)
Molecular Weight 5,039.244
Charge 4+
Protein IGHG4 (P01861)
Spectrum File MS_48_UO1_Erlic_E8
Method Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction SAX-ERLICH Enrichment
Sample Human Serum

Transitions

Exp. R.T. Peak R.T. Norm. R.T. Prec. m/z Prec. z Prod. m/z Prod. z Label %Int
60.833 60.836 119.024 1,256.316 4+ 1,456.010 3+ Y[pH4N3F] 82.7
60.833 60.836 119.024 1,256.316 4+ 1,401.992 3+ Y[pH3N3F] 33.7
60.833 60.836 119.024 1,256.316 4+ 1,577.720 3+ Y[pH5N4F] 16.7
60.833 60.836 119.024 1,256.316 4+ 1,280.281 3+ Y[pH2N2F]* 6.2
60.833 60.836 119.024 1,256.316 4+ 1,407.324 3+ Y[pH4N3] 5.6
60.833 60.836 119.024 1,256.316 4+ 1,334.299 3+ Y[pH3N2F]* 5.4
60.833 60.836 119.024 1,256.316 4+ 1,656.326 2+ Y[pNF]* 5.4

Spectra

14640;EThcD;60.827;14635;1256.320;Byonic:q-value=1.28e-05/GPS:MinICScore=6.605
14642;HCD;60.833;14635;1256.320;