TG003457

Peptide	R.EEQFN[H4N4F]STFRVVSVLTVVHQDWLNGK.E (PE001294)
Sequence	EEQFNSTFRVVSVLTVVHQDWLNGK
Glycans(s)	H4N4F (N5)
Mod(s)
Molecular Weight	4,556.085
Charge	4+
Protein	IGHG2 (P01859)
Spectrum File	MS_48_UO1_Erlic_E8
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	SAX-ERLICH Enrichment
Sample	Human Serum

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
53.632	53.699	100.311	1,135.526	4+	1,391.989	3+	Y[pH3N3F]	84.6
53.632	53.699	100.311	1,135.526	4+	1,270.278	3+	Y[pH2N2F]*	16.6
53.632	53.699	100.311	1,135.526	4+	1,324.296	3+	Y[pH3N2F]*	12.4
53.632	53.699	100.311	1,135.526	4+	1,641.320	2+	Y[pNF]*	9.8
53.632	53.699	100.311	1,135.526	4+	1,568.291	2+	Y[pN]	9.8
53.632	53.699	100.311	1,135.526	4+	1,343.303	3+	Y[pH3N3]	8.8
53.632	53.699	100.311	1,135.526	4+	1,216.260	3+	Y[pHN2F]*	7.2
53.632	53.699	100.311	1,135.526	4+	1,904.913	2+	Y[pH2N2F]*	6.5
53.632	53.699	100.311	1,135.526	4+	1,162.243	3+	Y[pN2F]*	6.0
53.632	53.699	100.311	1,135.526	4+	1,094.550	3+	Y[pNF]*	5.4
53.632	53.699	100.311	1,135.526	4+	1,289.285	3+	Y[pH2N3]	5.4
53.632	53.699	100.311	1,135.526	4+	1,095.013	4+	Y[pH3N4F]	5.2
53.632	53.699	100.311	1,135.526	4+	1,167.574	3+	Y[pHN2]	5.1

Spectra

12329;EThcD;53.510;12322;1135.527;Byonic:q-value=1.66e-09/GPS:MinICScore=191.199
12331;HCD;53.516;12322;1135.527;
12403;EThcD;53.740;12397;1136.036;Byonic:q-value=7.31e-10/GPS:MinICScore=274.896
12405;HCD;53.747;12397;1136.036;