TG000436

Peptide	R.SPYEM[Ox]FGDEEVMCLNGN[H5N4S2]WTEPPQCK.D (PE000168)
Sequence	SPYEMFGDEEVMCLNGNWTEPPQCK
Glycans(s)	H5N4S2 (N17)
Mod(s)	15.995 (M5), 57.021 (C13), 57.021 (C24)
Molecular Weight	5,256.012
Charge	4+
Protein	CFH (P08603), CFHR1 (Q03591)
Spectrum File	MS_24_UO1_CFH_UO1
Method	Orbitrap Fusion-Lumos, DDA (HCD pd EThcD), nano LC-MS/MS
Analytical Fraction	CFH Protein Standard
Sample	Human Serum

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
61.233	63.427		1,310.514	4+	1,619.175	2+	Y[pN]	45.9
61.233	63.427		1,310.514	4+	1,882.767	2+	Y[pH2N2]	16.4
61.233	63.427		1,310.514	4+	1,963.793	2+	Y[pH3N2]	14.6
61.233	63.427		1,310.514	4+	1,801.741	2+	Y[pHN2]	5.8
61.233	63.427		1,310.514	4+	1,720.714	2+	Y[pN2]	5.1

Spectra

17127;HCD;57.840;17122;1310.502;Byonic:q-value=2.65e-06/GPS:MinICScore=15.909
17128;EThcD;57.844;17122;1310.502;
17418;HCD;58.669;17415;1310.502;Byonic:q-value=6.89e-06/GPS:MinICScore=116.567
17420;EThcD;58.673;17415;1310.502;
17433;HCD;58.719;17430;1310.751;Byonic:q-value=1.09e-05/GPS:MinICScore=125.251
17435;EThcD;58.722;17430;1310.751;
17586;HCD;59.197;17576;1310.505;Byonic:q-value=0.00141/GPS:MinICScore=40.958
17588;EThcD;59.201;17576;1310.505;
17727;HCD;59.670;17725;1310.501;Byonic:q-value=6.93e-06/GPS:MinICScore=77.215
17728;EThcD;59.675;17725;1310.501;
17859;HCD;60.078;17858;1310.504;Byonic:q-value=2.66e-05/GPS:MinICScore=70.010
17861;EThcD;60.084;17858;1310.504;
18113;HCD;60.863;18111;1310.753;Byonic:q-value=2.7e-05/GPS:MinICScore=81.054
18115;EThcD;60.867;18111;1310.753;
18187;HCD;61.100;18170;1310.506;Byonic:q-value=2.71e-05/GPS:MinICScore=27.047
18189;EThcD;61.104;18170;1310.506;
18291;HCD;61.367;18289;1311.003;Byonic:q-value=2.71e-05/GPS:MinICScore=131.282
18292;EThcD;61.371;18289;1311.003;
18373;HCD;61.635;18372;1310.503;Byonic:q-value=2.73e-05/GPS:MinICScore=70.494
18374;EThcD;61.641;18372;1310.503;
18410;HCD;61.766;18408;1311.004;Byonic:q-value=2.74e-05/GPS:MinICScore=67.780
18412;EThcD;61.770;18408;1311.004;
18414;HCD;61.778;18413;1310.757;Byonic:q-value=2.75e-05/GPS:MinICScore=104.690
18416;EThcD;61.785;18413;1310.757;
18528;HCD;62.114;18526;1310.998;Byonic:q-value=7.27e-05/GPS:MinICScore=246.308
18530;EThcD;62.118;18526;1310.998;
18630;HCD;62.428;18628;1310.748;Byonic:q-value=2.76e-05
18632;EThcD;62.431;18628;1310.748;
18692;HCD;62.613;18691;1311.005;Byonic:q-value=1.1e-05/GPS:MinICScore=139.768
18694;EThcD;62.620;18691;1311.005;
18806;HCD;62.970;18802;1310.754;Byonic:q-value=2.78e-05/GPS:MinICScore=26.959
18808;EThcD;62.978;18802;1310.754;