TG000316

Peptide	R.TEVSSNHVLIYLDKVSN[H5N4FS2]QTLSLFFTVLQDVPVRDLKPAIVK.V (PE000239)
Sequence	TEVSSNHVLIYLDKVSNQTLSLFFTVLQDVPVRDLKPAIVK
Glycans(s)	H5N4FS2 (N17)
Mod(s)
Molecular Weight	6,994.388
Charge	6+
Protein	A2M (P01023)
Spectrum File	MS_31_UO1_MARS_MS2_1ug_longn_CE20_std
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	MARS Depleted
Sample	Human Serum

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
81.548	81.401		1,163.737	6+	1,208.164	4+	Y[pN]	31.2
81.548	81.401		1,163.737	6+	1,339.961	4+	Y[pH2N2]	23.1
81.548	81.401		1,163.737	6+	1,610.550	3+	Y[pN]	20.9
81.548	81.401		1,163.737	6+	1,380.474	4+	Y[pH3N2]	17.7
81.548	81.401		1,163.737	6+	1,376.475	4+	Y[pH2N2F]*	17.2
81.548	81.401		1,163.737	6+	1,416.988	4+	Y[pH3N2F]*	17.1
81.548	81.401		1,163.737	6+	1,508.271	4+	Y[pH4N3F]	12.6
81.548	81.401		1,163.737	6+	1,467.758	4+	Y[pH3N3F]	11.5
81.548	81.401		1,163.737	6+	1,299.448	4+	Y[pHN2]	11.4
81.548	81.401		1,163.737	6+	1,206.819	5+	Y[pH4N3F]	11.1
81.548	81.401		1,163.737	6+	1,431.244	4+	Y[pH3N3]	10.9
81.548	81.401		1,163.737	6+	1,244.679	4+	Y[pNF]*	9.0
81.548	81.401		1,163.737	6+	1,177.607	5+	Y[pH4N3]	8.9
81.548	81.401		1,163.737	6+	1,157.395	4+	Y[p]	8.2
81.548	81.401		1,163.737	6+	1,145.197	5+	Y[pH3N3]	6.7
81.548	81.401		1,163.737	6+	1,542.857	3+	Y[p]	6.4
81.548	81.401		1,163.737	6+	1,659.236	3+	Y[pNF]*	6.3
81.548	81.401		1,163.737	6+	1,335.962	4+	Y[pHN2F]*	6.0
81.548	81.401		1,163.737	6+	1,174.408	5+	Y[pH3N3F]	5.9

Spectra

24877;EThcD;81.308;24861;1163.905;Byonic:q-value=2.42e-13/GPS:MinICScore=82.664
24879;HCD;81.314;24861;1163.905;
25041;EThcD;81.778;25031;1163.908;Byonic:q-value=5.29e-12/GPS:MinICScore=50.370
25043;HCD;81.783;25031;1163.908;