TG000264

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Peptide R.LSLHRPALEDLLLGSEAN[H5N4FS]LTCTLTGLR.D (PE000154)
Sequence LSLHRPALEDLLLGSEANLTCTLTGLR
Glycans(s) H5N4FS (N18)
Mod(s) 57.021 (C21)
Molecular Weight 5,040.336
Charge 4+
Protein IGHA1 (P01876), IGHA2 (P01877)
Spectrum File MS_31_UO1_MARS_MS2_1ug_longn_CE20_std
Method Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction MARS Depleted
Sample Human Serum

Transitions

Exp. R.T. Peak R.T. Norm. R.T. Prec. m/z Prec. z Prod. m/z Prod. z Label %Int
58.056 58.068 1,256.592 4+ 1,583.849 2+ Y[pN] 42.2
58.056 58.068 1,256.592 4+ 1,456.378 3+ Y[pH4N3F] 15.8
58.056 58.068 1,256.592 4+ 1,920.471 2+ Y[pH2N2F]* 12.3
58.056 58.068 1,256.592 4+ 1,656.878 2+ Y[pNF]* 8.5
58.056 58.068 1,256.592 4+ 1,402.361 3+ Y[pH3N3F] 7.9
58.056 58.068 1,256.592 4+ 1,847.442 2+ Y[pH2N2] 17.6
58.056 58.068 1,256.592 4+ 1,407.692 3+ Y[pH4N3] 15.4
58.056 58.068 1,256.592 4+ 1,928.468 2+ Y[pH3N2] 14.9
58.056 58.068 1,256.592 4+ 1,353.675 3+ Y[pH3N3] 9.7
58.056 58.068 1,256.592 4+ 1,056.235 3+ Y[pN] 7.2
58.056 58.068 1,256.592 4+ 1,482.310 2+ Y[p] 6.8
58.056 58.068 1,256.592 4+ 1,685.389 2+ Y[pN2] 5.3
58.056 58.068 1,256.592 4+ 1,766.415 2+ Y[pHN2] 5.3

Spectra

16147;EThcD;56.802;16135;1256.841;Byonic:q-value=1.89e-13/GPS:MinICScore=149.876
16150;HCD;56.808;16135;1256.841;
16587;EThcD;58.051;16576;1257.087;Byonic:q-value=1.1e-07/GPS:MinICScore=138.624
16589;HCD;58.056;16576;1257.087;
16651;EThcD;58.225;16633;1256.845;Byonic:q-value=2.88e-05/GPS:MinICScore=290.515
16653;HCD;58.231;16633;1256.845;