TG000227

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Peptide R.LSLHRPALEDLLLGSEAN[H5N4S2]LTCTLTGLR.D (PE000002)
Sequence LSLHRPALEDLLLGSEANLTCTLTGLR
Glycans(s) H5N4S2 (N18)
Mod(s) 57.021 (C21)
Molecular Weight 5,185.374
Charge 6+
Protein IGHA1 (P01876), IGHA2 (P01877)
Spectrum File MS_31_UO1_MARS_MS2_1ug_longn_CE20_std
Method Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction MARS Depleted
Sample Human Serum

Transitions

Exp. R.T. Peak R.T. Norm. R.T. Prec. m/z Prec. z Prod. m/z Prod. z Label %Int
63.197 63.234 862.237 6+ 1,583.849 2+ Y[pN] 43.5
63.197 63.234 862.237 6+ 1,056.235 3+ Y[pN] 26.3
63.197 63.234 862.237 6+ 1,482.310 2+ Y[p] 13.3
63.197 63.234 862.237 6+ 1,231.964 3+ Y[pH2N2] 11.7
63.197 63.234 862.237 6+ 1,285.981 3+ Y[pH3N2] 7.4
63.197 63.234 862.237 6+ 786.712 6+ Y[pH4N4S] 5.9
63.197 63.234 862.237 6+ 1,123.929 3+ Y[pN2] 5.8
63.197 63.234 862.237 6+ 1,177.946 3+ Y[pHN2] 5.3

Spectra

18438;EThcD;63.079;18436;862.236;Byonic:q-value=0.0005/GPS:MinICScore=229.503
18440;HCD;63.088;18436;862.236;
18520;EThcD;63.300;18516;862.403;Byonic:q-value=2.18e-05/GPS:MinICScore=88.230
18522;HCD;63.306;18516;862.403;