US HUPO: Introduction to Bottom-Up Proteomics Informatics (2011)
Sunday, March 20, 2011, 9:00 am - 12:00 pm
Nathan Edwards (Georgetown)
This class will provide an introduction to the informatics of protein identification from bottom-up/shotgun proteomics workflows. The class is intended for applied laboratory or computational researchers seeking basic, practical instruction in the analysis of peptide tandem mass-spectra datasets. Topics will include: search engines, protein sequence databases, and statistical significance of search results.
Advanced topics, such as proteogenomics, meta-search, and result combining may be covered as time and interest permits. The class will conclude with a hands-on tandem mass-spectrometry analysis exercise on participants' laptop computers.
- Protein Identification by Sequence Database Search.
- Protein Sequence Databases, Peptides to Proteins, and Statistical Significance.
- Protein Identification by Peptide Mass Fingerprint.
- Protein Identification by Sequence Database Search with Tandem Mass Spectra.
Download spectra (Right-click and select "Save Link As...")
- Protein Identification by Sequence Tags.